VO Biochip Technologies in (Bio)Analytical Chemistry

Prüfungsinformationen

schriftlich

Lernaufwand

Beschreibung

To give an overview of current biochip technolgies, different platforms and possibly applications. In addition we will present new developments in the field of DNA/Protein and Cell Chips.

1. Modul: Introduction and Basics (G. Haberhauer) - Introduction to the structure of the lecture - Micro Arrays & Biochips - Definition - Area of Applications - Overview of different array systems - Analytical concepts & technologies - Theoretical limits of the approaches - Short introduction to the following moduls 2. Modul: DNA & Protein Chips - Surface and detection systems (C. Preininger) - surfaces & immobilization o substrates (glass, plastic, metal) and substrate activation o surface functionalization (reactive polymers and particles; coating methods; nanostructuring; 1D (i.E. SAMs) vs. 2D (i.E. PEG) vs. 3D (i.E. hydrogels)) o Immobilization methods (binding principles, differences between DNA and proteins) o Interaction between immobilized biomolecule and surface o Surface requirements with respect to detection system and on-chip assay - detection - optical systems o fluorescence o (electro)chemiluminescence o surface plasmon resonance o labels: indicators, liposomes, nanoparticles, quantum dots 3. Modul: DNA Chips - Marker identification & applications (A.Sessitsch) - Diagnostic chips and requirements; - molecular/phylogenetic markers, - Probe design - Short and long oligonucleotide probes - Diversity arrays; - Spezifity and Sensitivity - Examples of use 4. Modul: Cell Chips (P. Ertl) o what is µTAS, history and overview (from concept to µTAS) o microfluidics for cell cultures (theoretical aspects and components) o cell patterning techniques (microcontact, microfluidic, stencil patterning) o cell sampling, manipulation and treatment (trapping, sorting, lysis, transfection, fusion etc. ) o cell analysis (electrical, optical, magnetic etc.) o single cell analysis (ion channel studies etc.) o cell culture analogues (microphysiometers, neuronal biosensors etc.) o future prospects (integration of nanotechnology)

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